Gadvasu

Faculty Detail

Academic Credentials

Teaching and Other Appointments

Field of Specialization: Stem cell and Molecular biology, Bioinformatics

Teaching various UG and PG courses of College of Animal Biotechnology pertaining to Cell and Molecular Biology, Bioinformatics and Animal Biotechnology.

Other Appointments

• Member, PME cell (COABT)
• Member, IPR cell (COABT)
• Member sec. Budget, purchase/physical verification committee, COABT
• Store in charge- COABT

Area of Research Interests

Our laboratory, Animal Stem Cells lab, is working on two aspects of stem cell biology,

1) Exploring strategies to increase milk production in dairy animals by increasing mammary stem cell population using nucleotide analog, to increase cell turnover and lactation persistency

2) Developing regenerative therapy in canines using mesenchymal stem cell and cell-derived products. One of the approaches that we are interested in is the in-situ amelioration of mammary stem/progenitor cells. We are attempting to establish the application of stem cells and cell-based products for therapeutic application in animal diseases namely, mastitis, and wound healing. Finding an alternative therapy of mastitis without the use of antimicrobials and enhancing tissue regeneration using cell-based therapy are our long-term goals of the research.

Ongoing Projects

SN	Name/ No of Project/ Scheme	Funding agency	Period From To	Scientists involved   (PI/ Co-PI)	Total Funding
1.	Investigating the Role of Adipose Tissue-Derived Stromal Vascular Fractions (SVFs) Combined with Xanthosine in Veterinary Regenerative Medicine	DBT	2022-2025	PI: Dr. Ratan Kumar Choudhary CoPI; Dr. Yashpal Singh Dr. Ashwani Kumar	49.99 Lakh
2.	Immunomodulation  potentials of apoptotic  mesenchymal stem cells	SERB-SURE	26-12-2023-26-12-2025	PI: Dr. Ratan Kumar Choudhary	23.56 Lakh
3.	Modelling of indigenous diagnostics and immunopotent vaccine candidates to combat African swine fever in India	DBT	25/03/2021- 25/03/2024	PI: Yashpal S Malik Co-PI: Dr. Ratan Kumar Choudhary Dr. BV Sunil Kumar Dr. Niraj Singh	39.56 Lakh

Completed Projects

SN	Name/ No of Project/ Scheme	Funding Agency	Period From   To	Total Funding	Scientist involved (PI/ Co-PI)
1.	Elucidating the role of Cas6, Cas7 and Cas8 in spirochetes CRISPR adaptive immunity against alien genetic elements	DBT- NER, New Delhi	2017-2021	26.0 Lakhs (released 13 Lakhs)	PI: IIT Guwahati CCPI from GADVASU   Dr. Ratan Kumar Choudhary CoPI from GADVASU: Dr. Satparkash Singh
2.	Fast clotting clinical grade hemostatic agent for emergency care	DST-SERB	2018-2022	--	PI and other centers CoPI NIT Rourkela   Co-PI from GADVASU: Dr. Ratan Kumar Choudhary Dr. Devendra Pathak

Research Honour’s /Awards

• Guru Angad Dev Veterinary and Animal Sciences University “Appreciation Award for Research” for brining extra-mural funds for the basic research to the university – 2018.
• Poster award at International Conference of Society for Bioinformatics and Biological Sciences (SBBS), SKAUST, Jammu, India; October 12-13.
• Winner of Vector Lab’s (USA) photomicrography contest – 2017 (https://vectorlabs.com/uk/gallery/contest.html)
• Poster Award at International Conference on Reproductive Health with Emphasis on Strategies for Infertility, Assisted Reproduction and Family Planning (ISSRF-2017), AIIMS, New Delhi, Jan 23-25 2017.
• International Travel Award by DST, New Delhi – 2015
• Second Prize Winner at BARC poster day at United States Department of Agriculture (USDA), Beltsville, Maryland- 2012
• First Prize Winner PhD Division at the Joint Annual Meeting of the American Dairy Science Association (ADSA) and American Animal Science Association (ASAS) at New Orleans- 2011
• Novus International Inc. Travel Award to 63rd Annual EAAP meeting at Bratislava, Slovakia– 2011
• Second Prize Winner of Oral presentation, University of Maryland- 2011
• Third Prize Winner at BARC poster day USDA, Beltsville-2011
• R. F. Davis Scholarship for outstanding academic record, research and commitment to agriculture and natural resources, University of Maryland – 2011
• Dean’s Fellowship, University of Maryland, College Park, Maryland – 2011
• First Prize Winner of poster presentation, University of Maryland, College Park-2011
• J. K. Goldhaber Travel Award, University of Maryland- 2010
• Guru Angad Dev Veterinary and Animal Sciences University “Appreciation Award for Research” for brining extra-mural funds for the basic research to the university – 2018.
• Poster award at International Conference of Society for Bioinformatics and Biological Sciences (SBBS), SKAUST, Jammu, India; October 12-13.
• Winner of Vector Lab’s (USA) photomicrography contest – 2017 (https://vectorlabs.com/uk/gallery/contest.html)
• 2017 Poster Award at International Conference on Reproductive Health with Emphasis on Strategies for Infertility, Assisted Reproduction and Family Planning (ISSRF-2017), AIIMS, New Delhi, Jan 23-25 2017.
• International Travel Award by DST, New Delhi – 2015
• Second Prize Winner at BARC poster day at United States Department of Agriculture (USDA), Beltsville, Maryland- 2012
• First Prize Winner PhD Division at the Joint Annual Meeting of the American Dairy Science Association (ADSA) and American Animal Science Association (ASAS) at New Orleans- 2011
• Novus International Inc. Travel Award to 63rd Annual EAAP meeting at Bratislava, Slovakia– 2011
• Second Prize Winner of Oral presentation, University of Maryland- 2011
• Third Prize Winner at BARC poster day USDA, Beltsville-2011
• R. F. Davis Scholarship for outstanding academic record, research and commitment to agriculture and natural resources, University of Maryland – 2011
• Dean’s Fellowship, University of Maryland, College Park, Maryland – 2011
• First Prize Winner of poster presentation, University of Maryland, College Park-2011
• J. K. Goldhaber Travel Award, University of Maryland- 2010

No. of Publications

Research: 59	Extension: 06	Books: 06	Manuals: 08     Book Chapter:08

Publications

• Sahoo B, Choudhary, R. K., Sharma P, Choudhary S and Gupta MK. 2021. Significance and Relevance of Spermatozoal RNAs to Male Fertility in Livestock. Frontiers in Genetics. 12:768196. (IF-4.599).
• Choudhary, R. K and   Zhao, F-Q. 2022. Invited Review: Stem cells in mammary health and milk production. Current Stem Cell Research and Therapy. 17(3):207-213 (IF-3.758)
• Choudhary, R. K. and Capuco, A. V. 2021. Expression of NR5A2, NUP153, HNF4A, USP15 and FNDC3B is consistent with their use as novel biomarkers for mammary stem/progenitor cells. Molecular Histology. 52(2):289-300. (IF-2.35).
• Spitzer, A., Tian, Q., Choudhary, R. K. and Zhao, F-Q. 2020. Bacterial endotoxin induces oxidative stress and reduces milk protein expression and hypoxia in the mammary gland. Oxidative Medicine and Cellular Longevity. 24;2020: 3894309. (IF-4.868).
• Shangraw, E., Rodrigues, R., Witzke, M., Linder, H. F., Choudhary, R. K., Spitzer, A., Zhao, F.-Q., McFadden, T. B. 2020. Intramammary lipopolysaccharide infusion elicits local or systemic effects depending on milk component. Journal of Dairy Science. 103(8):7487-7497. (IF-4.034)
• Capuco, A. V. and Choudhary, R. K. 2020. Invited Review: Determinants of milk production: Understanding population dynamics in the bovine mammary epithelium. Journal of Dairy Science. 103(3):2928-2940.  (IF-4.034)
• Choudhary R. K., Choudhary, S., Mukhopadhyay, C. S., Pathak, D. and Verma, R. 2019. Deciphering the transcriptome of prepubertal buffalo mammary glands using RNA sequencing. Functional & Integrative Genomics. 19(2):349-362. (IF-3.41)
• Choudhary, S., Li, W., Bickhart, D., Verma, R., Sethi, R. S., Mukhopadhyay, C. S. and Choudhary, R. K. 2018. Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology. Journal of Animal Science and Technology. 60:18. (IF-1.95)
• Choudhary, R. K., and Capuco, A. V. 2012. In vitro expansion of mammary stem/progenitor cell population by xanthosine treatment. BMC Molecular and Cell Biology. 13:14. (IF-2.81)
• Choudhary, R. K., Li, R. W., Evock-Clover, C. M. and Capuco, A. V. 2013. Comparison of transcriptomes of long-term label-retaining-cells and control cells microdissected from mammary epithelium: An initial study to characterize potential stem/progenitor cells and identify possible biomarkers. Frontiers in Oncology. 3 (21). (IF-6.244)

Authored/Edited Books:

• Choudhary, R. K. and Choudhary, S. 2021. Stem Cells in Veterinary Science. Hard Cover ISBN: 978-981-16-3463-5.  Pp. XII, 375. (editor) https://link.springer.com/book/10.1007/978-981-16-3464-2?page=1#toc 
• Mukhopadhyay, C. S., Choudhary, R. K. and Iquebal, M. A. 2017. Basic Applied Bioinformatics, Wiley. ISBN: 978-1-119-24433-2. (editor) https://www.wiley.com/en-us/Basic+Applied+Bioinformatics-p-9781119244332
• Choudhary R. K and Malik Y S. 2021. Biotechnological Approaches in Animal Research and Disease Diagnosis. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab. ISBN 978-93-5566-112-8. Pp. 106- (editor)
• Malik, Y. P. S., Sethi, R. S. Deepti, N., Choudhary, R. K., Pathak, D., Mehta, N. and Shanthanagouda, A. H. (2022). Transforming Livestock Economy through Innovations in Immunology and Biotechnology: A Souvenir-cum-Compendium. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab. ISBN 978-93-5619-958-3. Pp. 250.- (editor).