Epidemiological studies/data on ixodid ticks in bovines, gastrointestinal parasites of large and
small ruminants and swines, haematozoan
parasites of bovines, equines, canines of various agro-climatic zones of
Punjab.
Diagnosis:
Detection
of Trypanosoma evansi, Anaplasma marginale, Babesia bigemina,
Theileria annulata and Cryptosporidium spp in large animals by conventional and nested PCR
assays.
Detectionof
Ehrlichia canis, Babesia gibsoni, Babesia vogeli,
Hepatozoon canis in canines by conventional, nested PCR techniques.
Theileria
equi, T. evansi and H. canis by conventional, nested PCR
& LAMP
Detection
of haemoaparasites viz Theileria
equi, T. annulata, A. marginale, B. bigemina and H.
canis in ixodid tick vectors.
Characterization
of Eimeria spp. in swine by conventional and nested PCR assays.
Multiplex
PCR assay for simultaneous detection of T.evansi,
T. annulata and B. bigemina in bovines, T.
equi and T. evansi in the equines, Ehrlichia canis,
B. gibsoni, B. vogeli, Hepatozoon canis in canines.Taqman singleplex based real time PCR
for theileriosis, trypanosomosis and babesiosis in bovines.
Multiplex
SYBR Green based real-time PCR assay standardized and evaluated to simultaneously
detect the parasitic DNAs of B. vogeli & E. canis and B. gibsoni &
H. canis in dogs.
Molecular
characterization of economically important gastrointestinal (Cryptosporidium spp, Eimeria spp., Ancylostoma spp.), haemoparasites (Babesia spp., Theileria spp.,
Ehrlichia spp. Hepatozoon spp., T. evansi)
of livestock and pet animals.
Immunodiagnosis
of paramphistomosis, toxocarosis, fasciolosis, ehrlichiosis, babesiosis,
cryptosporidiosis, trypanosomosis
Detection
of resistance:
Acaricide resistance status in Rhipicephalus
microplus and Hyalomma anatolicum based on Adult
Immersion Test (AIT) against synthetic
pyrethroids (cypermethrin and deltamethrin)
revealed higher resistance levels against deltamethrin.
Pioneer
report of ivermectin resistance from India in Rhipicephalus microplus
from Punjab by Larval Immersion test (LIT) showed resistance status in all
field isolates.
Standardization and application of
modified larval packet test (LPT) using nylon membranes as substrate and
formulated amitraz for detection of amitraz resistance in Hyalomma
anatolicum and Rhipicephalus microplus showed low levels of
resistance from Punjab.
Detection of
anthelmintic resistance by FECRT against ivermectin, levamisole, morantel
citrate and fenbendazole showed multiple drug resistance in small ruminants of
Punjab.
Detection of benzimidazole resistance by molecular based allele specific PCR in adult and larvae of
Haemonchus contortus in sheep and goats of different zones of Punjab
showed great level or resistance.
Screening of indigenous plants for evaluation of their acaricidal
properties:
Assessment of the acaricidal activity
of various extracts of aqueous of Murraya koenigii, Cymbopogon
winterianus, Vitex negundo, and Withania somnifera along with
roots of V. negundo, Dalbergia sissoo
showed better acaricidal activities against the synthetic pyrethroids resistant
R. microplus ticks.
Alcoholic and various aqueous extracts
of Piper longum, Piper nigrum and Zingiber officinale and
their combinations against Hyalomma anatolicum revealed highest
acaricidal property in the alcoholic extract of P. longum seeds than Zingiber
officinale.
In vitro
acaricidal activity evaluation of essential oils (EOs) of cloves (Syzygium
aromaticum), bark of cinnamon (Cinnamomum zeylanicum), leaves of
lemon grass (Cymbopogon citratus) and their combinations against R.
microplus by LPT revealed highest acaricidal activity in cinnamon EO.
Control:
Phytochemical
control of Eimeria tenella infection
in poultry birds, anticoccidial studies in small ruminants, Immunomodulatory
and pharmacokinetics of drugs in T.
evansi experimentally infected animals, efficacy of flumethrin, deltamethrin and amitraz for tick control in
buffaloes.
