Gadvasu

Development and optimization of dystocia handling procedure

• Sharma’s modified detorsion method in buffaloes.
• Cervical dilatation by massage using carboxymethyl cellulose gel as a non-hormonal approach.
• Use of hyaluronidase for hastening cervical dilatation.
• Management of dehydration in dystocia cases with oral rehydration along with intravenous administration of hypertonic saline and Dextran.
• Alleviating post caesarean uterine adhesion formation using ‘Interceed’.

Management of infertility

• Uterine cytobrush cytology for the diagnosis of subclinical endometritis.
• Intrauterine proteolytic enzyme therapy for subclinical endometritis.
• Treatment of uterine infections through immunomodulator (E coli LPS).
• N Acetyl cysteine (NAC) therapy in mares affected with uterine infection.
• Induction of ovarian cyclicity and ovulation in summer anoestrous buffalo using melatonin implants.
• Induction of follicular wave emergence through ultrasound guided follicular ablation and estradiol 17-beta administration in buffalo.
• Transvaginal ultrasound guided cyst ablation as a non-hormonal method for repeat breeding cows suffering from cystic ovarian follicles.

Estrus synchronization and ovulation

• Treatment of repeat breeding through Ovsynch plus CIDR-based fixed-time AI protocol.
• Doublesynch and Estradoublesynch protocols for inducing cyclicity and improving pregnancy rates in anestrous buffaloes.
• Modified Heatsynch and Presynch-Heatsynch protocols for inducing cyclicity during summer in buffalo.
• Estradoublesynch and progesterone based ovsynch protocols for delayed pubertal buffalo heifers.

Semen Technology

• Sephadex filtration of semen to improve semen quality and fertility.
• Rapid Freezing protocol for cryopreservation of Buffalo bull semen.
• Identification of fertility associated metabolites in seminal plasma.
• Artificial insemination in gilts/sows using golden sow catheter.
• Ice recrystallization inhibitors to improve post-thaw semen quality in buffalo bulls.
• Graphene oxide as a potential cryoprotectant for freezing of bovine semen.

Embryo transfer technology

Evaluated effects of serum metabolites on superovulatory response and embryo production in Sahiwal cows.
• Assessed developmental competence of in vitro & in vivo matured oocytes retrieved by ovum pick up in buffaloes.